Dr. Swain

Sunday
May 9

 

8:00 AM -

11:30 AM

BCC Ballroom I

BCC Ballroom II

• Juan Rivera, NIAMS/NIH, Sphingosine kinases and sphingosine-1-phosphate in mast cell biology and function
• Toshiaki Kawakami, La Jolla Institute for Allergy and Immunology, PLC-b3, a tumor suppressor, inhibits skin inflammation
• Soman N. Abraham, Duke University Medical Center, Mast cell modulation of adaptive immune responses to pathogens
• Jean S. Marshall, Dalhousie University, Mast cell responses to viral infection and Toll-like receptor-mediated activation
• Melissa A. Brown, Northwestern University, Roles of mast cells in autoimmunity
• Stephen J. Galli, Stanford University, Mast cells as negative regulators of innate and adaptive immune responses

• Thaddeus S. Stappenbeck, Washington University School of Medicine, Autophagy, Paneth cells and intestinal inflammation

• Richard S. Blumberg, Brigham & Women's Hospital/Harvard Medical School, ER stress and intestinal inflammation

• Andrew J. Macpherson, University of Bern, The continuum between adaptive and innate immunity in maintaining mutualism with commensal intestinal bacteria

• Bana Jabri, University of Chicago, TLR6 and immune regulation

• Christopher L. Karp, Cincinnati Children’s Hospital Research Foundation, Immunobiology of a gene modifying lung disease in cystic fibrosis

• Averil Ma, University of California, San Francisco, Regulation of NF-κB and NOD2 pathways in mucosal tissues

• Ellen Hsu, State University of New York, The basis of allelic exclusion?
• Martin F. Flajnik, University of Maryland at Baltimore, Immunoglobulins and gamma/delta T cell receptors: How do we decide?
• John P. Cannon, University of South Florida, Genetics, structure and function of novel immune receptors
• Max D. Cooper, Emory University, Evolution of alternative adaptive immune systems
• Thomas Boehm, Max-Planck-Institute of Immunobiology, Evolution of the thymus in vertebrates
• Roy A. Mariuzza, University of Maryland Biotechnology Institute, Structural insights into the evolution of adaptive immunity

AAI Block Party “A Night at the Yard” *
Generously supported by BioLegend
Sunday, May 9, 7:00 PM - 9:30 PM — Orioles Park at Camden Yards

Join us for this “Grand Slam” experience, supported by BioLegend at Camden Yards, home of the Baltimore Orioles! Camden Yards was built in the tradition of the magnificent big-league ballparks of the early 1900’s but with 21st century state-of-the-art technology.

Attention all graduate students and postdoctoral fellows! Join other young scientists for this hip networking event. You’ll enjoy a relaxed but rockin’ atmosphere to meet, network—and dance! Party includes snacks and beverages.

AAI Clinical Immunology Committee

AAI Committee on Public Affairs

Scientists who use animals in their research are faced with myriad challenges, ranging from the mundane (administrative burden) to the menacing (animal rights extremists). This session will explore legislative and legal challenges to the use of animals in research and provide invaluable advice about how researchers can protect themselves and their labs from animal rights extremists.

Meet the Advocacy Partners

Monday, May 10, 4:00 PM - 5:00 PM — BCC Room 328

Chair: John R. Schreiber, Tufts University School of Medicine; Chair, AAI Committee on Public Affairs

Representatives from the following groups will be available to talk about their work and about scientific and funding opportunities that may be available to AAI members/meeting attendees:

AAI Education Committee

• Sharon A. Stranford, Mount Holyoke College, Money and time: funding your research and balancing your job
• Richard A. Goldsby, Amherst College, Preparing for and finding a job
• Jennifer A. Punt, Haverford College, Innovative models for laboratory teaching
• Devavani Chatterjea, Macalester College, Engaging with the material: teaching reading, writing and questioning skills

AAI Education Committee &
AAI Committee on the Status of Women

Dr. Nacy

AAI Minority Affairs Committee

AAI Publications Committee

NATIONAL CANCER INSTITUTE (NCI)

• Mary M. Zutter, Vanderbilt University School of Medicine, a₂b₁ integrins in squamous cell carcinoma: inflammation and lymphangiogenesis

• Michael T. Lotze, University of Pittsburgh, Metabolism, inflammation, and cancer: role(s) of damage-associated molecular pattern molecules

• Hans Schreiber, University of Chicago, Inflammation and stroma as tumor promoters in cancer induction

• Klaus Ley, La Jolla Institute for Allergy and Immunology, Neutrophil recruitment to sites of inflammation

• James J. Lee, Mayo Clinic Arizona, The role(s) of eosinophils in cancer: pro-tumorigenic, anti-tumorigenic, or simply innocent bystanders?

• James W. Kazura, Case Western Reserve University, Exposure and age-related heterogeneity of naturally acquired immunity to human Plasmodium falciparum infection
• Myriam Arévalo-Herrera, Instituto de Inmunología de la Universidad del Valle, Plasmodium vivax malaria:  studies on transmission-blocking immunity in search for vaccine for human use
• Eleanor M. Riley, London School of Hygiene and Tropical Medicine, Long-lived antibody and B cell memory responses to the human malaria parasites, Plasmodium falciparum and Plasmodium vivax
• Douglas T. Golenbock, University of Massachusetts Medical School, Recognition of DNA by the innate immune system causes inflammation during malaria

• James E. Crowe, Jr., Vanderbilt University Medical Center, Molecular diversity in HIV-specific human B cell repertoires
• Andrea Cerutti, Mount Sinai School of Medicine, HIV infection and immunoglobulin class switching
• Robert T. Woodland, University of Massachusetts Medical Center, Humanized mice as a research tool for HIV vaccine development
• Garnett Kelsoe, Duke University Medical Center, Increasing immunogenicity of HIV MPER antigens by breaking tolerance

• Ann Marshak-Rothstein, University of Massachusetts Medical Center, Tolling down the road to SLE
• Donald Y. M. Leung, National Jewish Health, Role of barrier in the skin’s innate immune response
• Thirumala-Devi Kanneganti, St Jude Children's Research Hospital, NLR inflammasomes in innate immunity, inflammation, and host defense
• Daniel L. Kastner, NIAMS, NIH, Horror autoinflammaticus: the expanding spectrum of systemic autoinflammatory disease

National Institute on Aging (NIA)

• Susan L. Swain, Trudeau Institute, With age, increased naïve CD4 T cell lifespan, caused by reduced expression of Bim, is necessary for development of functional defects
• Nan-ping Weng, NIA, NIH, Probing the in vivo changes of human immune system with age: a 5-year study of the old participants from the Baltimore Longitudinal Study on Aging (BLSA)
• Jörg J. Goronzy, Stanford University, T cell signaling in aging - in favor of phosphatase activity
• Claire A. Chougnet, Cincinnati Children’s Hospital, Accumulation of regulatory T cells and age-related immunosuppression

• Marc K. Jenkins, University of Minnesota, Monitoring immune responses in vivo
• Anita S.F. Chong, University of Chicago, Memory B cells as barriers to transplant outcomes
• Mandy L. Ford, Emory University, Memory T cells as barriers to transplant outcomes
• Xian C. Li, Beth Israel Deaconess Medical Center/Harvard Medical School, Targeting memory T cells in transplantation

• Stephen D. Miller, Northwestern University, An antigen-specific tolerance approach for the therapy of autoimmune disease and tissue transplantation
• Roland M. Tisch, University of North Carolina, Chapel Hill, The use of soluble MHC-peptide dimers to suppress beta cell autoimmunity
• David S. Bradley, University of North Dakota, An alternative therapy for autoimmune diseases:  the potential of an immature dendritic cell vaccine for spontaneous polyarthritis
• Robert B. Nussenblatt, NEI, NIH, Oral tolerance and ocular inflammatory disease

• Andrew M. Lew, Walter and Eliza Hall Institute of Medical Research, Iatrogenic depletion of cross-presenters and a new role for CD40L on DC
• Kristen J. Radford, Mater Medical Research Institute, Human blood DC subsets and function
• Bruce W. S. Robinson, Sir Charles Gairdner Hospital, DC subsets and cross presentation in cancer
• Jose A. Villadangos, Walter and Eliza Hall Institute of Medical Research, Antigen presentation specializations in the DC network
• Li Wu, Walter and Eliza Hall Institute of Medical Research, Transcription factor PU.1 controls DC development through the dose-dependent regulation of Flt3

• Tracy Hussell, Imperial College London, Regulation of immune surveillance in the lung
• Eyal Raz, University of California, San Diego, Th17: canonical and non-canonical differentiation pathways
• Arne N. Akbar, University College London, Decreased cutaneous immunosurveillance after antigenic challenge during aging
• Andrew L. Mellor, Medical College of Georgia Immunotherapy Center, Enhancing immunosurveillance in chronic inflammatory diseases by manipulating IDO

• Jacques Robert, University of Rochester Medical Center, Comparative and evolutionary study of tumor immunity in the non-mammalian xenopus model
• Eva Bengtén, University of Mississippi Medical Center, Insights into teleost IgD function
• J. Oriol Sunyer, University of Pennsylvania, Convergent evolution of mucosal immunoglobulins of fish and mammals
• Brantley R. Herrin, Emory University, Antigen recognition by lamprey variable lymphocyte receptors
• L. Courtney Smith, George Washington University, Highly variable immune- response proteins (185/333) from the sea urchin

• Reen Wu, University of California, Davis, Regulation of airway mucosal immunity by IL-19
• Kerstin Wolk, University Hospital Charite, IL-20 and IL-22:  biology and role in disease
• Elizabeth A. Grimm, University of Texas, MD Anderson Cancer Center, IL-24, an IL-10 family cytokine with immunostimulatory and tumor suppressor functions
• Grant Gallagher, HUMIGEN, the Institute for Genetic Immunology, Regulation of interferon lambda-1 (IL-29) in airway epithelium

• Bruce Beutler, Scripps Research Institute, Genetic approaches to innate immunity
• Julie Magarian Blander, Mount Sinai School of Medicine, PRRs and antigen presentation
• Peter J. Murray, St. Jude Children’s Research Hospital, Signaling to arginine metabolism in intracellular pathogenesis
• Katherine A. Fitzgerald, University of Massachusetts Medical School, Immunobiology of DNA sensing pathways

SATURDAY, MAY 8

Monetizing Your Intellectual Property: Protecting Ideas That Generate Income

Knobbe Martens Olson & Bear LLP

10:00 AM – 11:00 AM, Room 311

Presenters: Mark Benedict, Ph.D., Partner, Knobbe Martens Olson & Bear LLP; Michael L. Fuller, Partner, Knobbe Martens Olson & Bear LLP

We will explore what types of research innovations are protectable and discuss practical tips for protecting these innovations while avoiding some common pitfalls that could compromise your intellectual property. We will also develop strategies for leveraging your intellectual property to generate entrepreneurial opportunities, personal revenue, and support for your ongoing research.

Moving Beyond Phenotyping—Novel Molecular Probes Reagents to Analyze Cellular Functions

Invitrogen Corporation

10:00 AM – 11:00 AM, Room 312

Presenter: Jeffrey Croissant, Ph.D., Senior Marketing Manager, Flow Cytometry Systems, Molecular Probes - Invitrogen Labeling and Detection Technologies, Eugene, Oregon

Invitrogen recently introduced a number of novel reagents and assays developed using Molecular Probes fluorescence detection technologies that enable researchers and clinicians to analyze cell function far beyond subset identification. This tutorial will present a number of cutting edge fluorescent reagents for flow cytometry, imaging, and high content screening, with practical examples and details. A diverse number of applications will be presented including: Cell Proliferation, Cell Cycle, Viability, Apoptosis, and Organelle Labeling for live cell imaging. The cell proliferation and cell cycle products to be highlighted include: CellTrace Violet Cell Proliferation Kit as a CFSE alternative for use with violet laser excitation, the use of Click-iT EdU technology and highly specific antibodies for BrdU to enable a simplified method of dual pulse labeling for imaging and flow cytometry, Premo FUCCI Cell Cycle Sensor using live cell imaging to view fluorescent changes as a cell progresses through the cell cycle and division, and Vybrant DyeCycle Ruby Stain for far red excitation live cell cycle analysis without cytotoxicity. Viability reagents include the amine-reactive dye LIVE/DEAD Fixable Yellow and the impermeant nucleic acid dye SYTOX AADvanced stain. Apoptosis products will introduce the F2N12S Membrane Asymmetry probe for adherent and suspension cells detected with violet laser excitation. For imaging and HCS applications an introduction to Organelle Lights Reagents using BacMam Delivery Technology will be given. These reagents can easily be combined with phenotypic markers to develop higher color panels allowing more information to be collected from every experiment.

Multicolor Flow Cytometry: Tips for Panel Design and Analysis of Intracellular Targets

eBioscience, Inc.

11:00 AM – 12:00 PM, Room 312

Presenter: Traci Zell, Ph.D., Scientific Applications Manager, eBioscience, Inc.

Flow cytometry is increasingly recognized as an indispensable technique for deciphering complex cellular processes and interactions in a variety of systems that model normal and disease states. Recent advances in both instrument technologies and new fluorochromes have made it possible to characterize both the phenotype and function of individual cells within complex populations. Topics to be covered include: instrument performance and impact on multicolor panels, tips for panel design, and appropriate controls to validate panels.

A New, Flexible Alternative to Traditional Research Methods

Accuri® Cytometers, Inc.

12:00 PM – 1:00 PM, Room 311

Presenter: Maria Dinkelmann, Ph.D., Laboratory Manager, Accuri® Cytometers, Inc.

This workshop will highlight the versatility of the Accuri C6 Flow Cytometer System by focusing on data analysis of research experiments including: single platform cell counting with viability, proliferation and cell cycle analyses, population and phenotypic characterization and multiplex bead analysis. With its minimal set-up requirements and simple-to-learn user interface, the affordable C6 is an attractive alternative to many traditional research methods. Refreshments provided.

Microplate-based Antibody Arrays: A New High Performance Multiplex Technology

R&D Systems, Inc.

12:00 PM – 1:00 PM, Room 312

Presenter: Kathy Brumbaugh, Ph.D., Manager, Intracellular Department, R&D Systems, Inc.

R&D Systems Mosaic ELISA and Proteome Profiler 96 Antibody Arrays utilize the traditional sandwich ELISA format to measure multiple secreted or signal transduction-related factors in a single microplate well. The assays require small sample sizes and utilize common laboratory camera systems for data acquisition. Learn how these arrays combine sensitivity, reproducibility, and throughput not found in other multiplex formats.

Simultaneous Correlation of Cytokine Production with Treg and Th17 Cell Proliferation

BD Biosciences

1:00 PM – 2:00 PM, Room 311

Presenter: Jurg Rohrer, Ph.D., R&D Director/Research Immunology, BD Biosciences

Functionally polarized CD4+ T cell subsets can be identified based on their distinctive patterns of cytokine secretion and transcription factor expression. As a signature cytokine, Th17 cells express high levels of interleukin-17A (IL-17A) whereas T regulatory cells (Treg) are characterized by the expression of the FoxP3 transcription factor. In the mouse, TGFβis required for the production of IL-17A, although additional

cytokines are also produced by Th17 cells, including Th1-like cytokines (IL-2, IFN-γand Th2-like cytokines (IL-4, IL-10). Using an in vitro polarization system we aimed to correlate cell cycle progression with cytokine production, FoxP3 expression, and cytokine-induced phosphorylation. Cell cycle progression was monitored using a cell proliferation dye, cytokine production was monitored by intracellular staining as well as by cytometric bead array (CBA), Treg were detected by FoxP3 staining, and phospho-proteins were detected using Phosflow technology. Multiple fixation and permeabilization conditions were required for resolution of the intracellular cytokines, FoxP3, and phospho-proteins. We were able to show that under the specific culture conditions, IL-17A appeared after two days and was restricted to newer generations of cells. During the earlier culture time points, IL-2 and IL-17A were coexpressed, but after several days in culture the expression became mutually exclusive. Analysis of Stat5 phosphorylation revealed heterogeneous responses to secreted cytokines, with newer generations of cells showing the most robust phosphorylation responses. These data demonstrate the wealth of information that can be obtained from such experiments when using multiple analysis tools. The experiments also underscore the importance of optimizing the protocols for using the reagents to obtain the widest possible data matrixes.

SUNDAY, MAY 9

Measuring Immune Function with Imaging Flow Cytometry

Amnis Corporation

10:00 AM – 11:00 AM, Room 311

Presenters: Patricia Fitzgerald-Bocarsly, Ph.D., UMDNJ; Jing Wang, Ph.D., Univ. Rochester; Michael Russell, Ph.D., Univ. Buffalo; Lynn Hassman, Univ. Virginia; Adrian Wiestner, Ph.D., NHLBI

Measurement of mechanisms of immune function with the ImageStream imaging flow cytometry will be explored through the experiences of several prominent speakers. A diverse range of topics will be covered, including immune response to viral infection, phagocytosis of fungal pathogens, trafficking of antigens, and effector mechanisms of therapeutic antibody-induced tumor clearance.

Acoustic Focusing Flow Cytometry—A New Breakthrough in Cell-by-Cell Analysis

Invitrogen Corporation

10:00 AM – 11:00 AM, Room 312

Presenter: Jeffrey Croissant, Ph.D., Senior Marketing Manager, Molecular Probes - Invitrogen Labeling and Detection Technologies, Eugene, Oregon

Acoustic cytometry is a new technology that replaces or partly replaces hydrodynamic focusing of cells or particles in flow cytometry with forces derived from acoustic radiation pressure. The ability to focus cells into a tight line without relying on hydrodynamic forces allows many possibilities outside the scope of conventional flow cytometry. Dilute samples can be processed quickly. Flow rates can be varied, allowing control of particle delivery parameters such as interrogation time and volumetric sample input rates. Recently, Life Technologies unveiled the Applied Biosystems Attune Acoustic Focusing Cytometer that implements this new form of particle delivery into a benchtop analyzer. In this talk, the application of acoustic cytometry from fundamental principles to specific details of its implementation in cell analysis systems will be presented. This will be followed by an introduction of the Attune Acoustic Focusing Cytometer, a new benchtop cytometer that delivers greater sensitivity, higher sample acquisition rates, and greater control than conventional flow cytometers.

Measuring Immune Function with Imaging Flow Cytometry

Amnis Corporation

11:00 AM – 12:00 PM, Room 311

Presenters: Patricia Fitzgerald-Bocarsly, Ph.D., UMDNJ; Jing Wang, Ph.D., Univ. Rochester; Michael Russell, Ph.D., Univ. Buffalo; Lynn Hassman, Univ. Virginia; Adrian Wiestner, Ph.D., NHLBI

Measurement of mechanisms of immune function with the ImageStream imaging flow cytometry will be explored through the experiences of several prominent speakers. A diverse range of topics will be covered, including immune response to viral infection, phagocytosis of fungal pathogens, trafficking of antigens, and effector mechanisms of therapeutic antibody-induced tumor clearance.

Antibody-based Detection of Cytokine Expression Profiles: Arrays of Possibilities

RayBiotech, Inc.

11:00 AM – 12:00 PM, Room 312

Presenter: Brett Burkholder, Director, Tech Support & Marketing, RayBiotech, Inc.

Cytokine biology is often too complex to unravel using traditional techniques of molecular biology. Cytokines commonly work synergistically or antagonistically with other cytokines, with overlapping functions and cross-talk among cytokines being the rules rather than the exceptions. Therefore, using traditional ELISA or Western-blot analysis to measure changes in just a few cytokines can give an incomplete picture of the role of these proteins in an experimental model. Cytokine antibody arrays are multiplexed assays that can measure changes in expression of hundreds of cytokines simultaneously, allowing researchers to obtain a more global perspective on cytokine expression compared to traditional approaches. An overview of cytokine array technologies will be presented, as well as specific examples of how cytokine antibody arrays can be used to identify biomarkers, key factors, and drug targets and to elucidate biochemical mechanisms and pathways.

Fast and Versatile Isolation of Highly Functional Regulatory T Cells

STEMCELL Technologies, Inc.

12:00 PM - 1:00 PM, Room 311

Presenter: Andy Kokaji, Ph.D., Scientist, STEMCELL Technologies

The first step towards quality regulatory T cell (Treg) research is the isolation of highly purified, functional cells. Learn how to obtain the highest purity Tregs directly from whole blood or PBMC in less time with STEMCELL’s new range of products for the rapid and efficient isolation of human Tregs. This workshop will demonstrate how you can get your desired population of Tregs based on expression of CD25, CD127, CD49d, and CD45RA, while saving hours on the isolation procedure. Purified cells are highly functional and ready for downstream Treg assays.

New Inflammation and Cytokine Research Technologies: From Expression to Mechanism

SABiosciences Corporation, a QIAGEN Company

12:00 PM – 1:00 PM Room 312

Presenter: Brian McNally, Associate Product Manager, SABiosciences

SABiosciences, a QIAGEN company, has developed pathway-focused tools for investigating inflammation, infection and autoimmunity. This seminar will present case studies that measured gene expression and pathway activity and yielded mechanistic insights into the underlying biological system. Learn how PCR Arrays, ELISArrays, Cignal Reporters and Biology-on-Array will enhance immunology research.

Control of the Inflammatory Response of Primary Human Macrophages by Microstructures and Surface Chemistry

eBioscience, Inc.

2:00 PM – 3:00 PM, Room 312

Presenter: Matthias Bartneck, Dipl.-Biol., Interdisciplinary Center for Clinical Research “BioMAT”

Fluorinated polymers and gold nanoparticles are regarded as biocompatible materials and therefore are used as model materials to study the inflammatory response of macrophages. Topics to be covered include expression of inflammation-relevant genes, determining phenotype using real-time PCR and flow cytometry, and quantifying multiple cytokines from cell culture using the eBioscience Flowcytomix multiple analyte detection system.

Sensitive Rare Cell Enumeration and Whole

Blood Analysis Using the MACSQuant Analyzer

Miltenyi Biotec, Inc.

3:00 PM – 4:00 PM, Room 311

Presenter: Colin deBakker, Ph.D., Flow Cytometry Specialist, Miltenyi Biotec, Inc.

Detection and enumeration of rare cells using a flow cytometer has limitations. To overcome some obstacles, the MACSQuant Analyzer was designed with unique features, including a pump-driven fluidic system and a MACS Cell Enrichment Unit for magnetic pre-enrichment of target cells. This workshop will illustrate the simplicity of detecting and enumerating rare cells with application for multiple cell sources, including whole blood.

MONDAY, MAY 10